Snakebite envenoming is a serious neglected tropical disease that affects ~5 million people worldwide annually, leading to ~400,000 amputations and >100,000 deaths. High-quality genome assemblies, combined with transcriptome-guided annotation can help identify the key venom components that need to be targeted for neutralizing antibody development. With this as an objective, we have generated high-quality, near-chromosomal genome assemblies of highly venomous medically relevant snakes from Asia, Africa and North America. We have used this information to generate antibodies using single-cell genomics technologies and characterized such antibodies for their ability to neutralize venom components. These antibodies when combined with existing anti-venom can help improve the existing anti-venom and treatment for snake bite patients.